Home Project-material PHYTOCHEMICAL ANALYSIS AND THE ANTIINFLAMMATORY ACTIVITIES OF DICHLOROMETHANE FRACTION OF METHHANOL EXTRACT OF CRATEVA ADANSONII

PHYTOCHEMICAL ANALYSIS AND THE ANTIINFLAMMATORY ACTIVITIES OF DICHLOROMETHANE FRACTION OF METHHANOL EXTRACT OF CRATEVA ADANSONII

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Abstract

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CHAPTER ONE

INTRODUCTION AIM AND OBJECTIVES

Inflammation is one of body’s natural ways of protecting

itself. It is also an essential reaction of the body to

infection. Too little of an inflammatory response, and the

body is unable to repel microbial invasions or heal

injuries. Too much of an inflammatory response, and the

immune system begins attacking the body’s own organs

eventually leading to chronic disease (Sears, 2000).

Anti-Inflammatory refers to the property of a

substance or treatment that reduces inflammation.

There are obvious clinical markers of inflammation which

include pain, redness, fever, swelling and loss of

function (Pai et al. 2004). However if inflammation is

allowed to continue unchecked it may results in

neurodegenerative disease or cancer (Pai et al. 2004). A

variety of safe and effective anti-inflammatory agents

are available including non-steroidal anti-inflammatory

drugs, corticosteroids.

13Non-steroidal anti-inflammatory drugs (NSAID are

usually indicated for the treatment of acute and chronic

inflammation. NSAIDS work by reducing the production

of prostaglandins (Bayness & Marek, 2005).

Prostaglandins are chemicals that promote

inflammation, pain and fever. The enzymes that produce

prostaglandins are phospholipaseA2, PGH2synthase and

Cyclooxygenase (Cox). There are two types of COX

enzymes, cyclooxygenase-1 (Cox-1) and

cyclooxygenase-2 (Cox-2). NSAIDS reduce the

production of prostaglandin by inhibiting COX enzymes

resultant effect is that, therefore, inflammation, pain

and fever are reduced. Some herbs and plants possess

anti-inflammatory and they include:

S/No Common Name

Botanical Name

1 Andiroba oil Carapa guianensis

It is rich in omega-3 fatty acids that promote skin healing from cuts and may slow growth of skin cells in psoriasis and age spots. It relieves pain and swelling

142 Alfalfa Medicago sativa L.

It serves as an oxidant in the blood stream. They are staple of salads and contain nutrient, the leaves hold the best healing potential and contain phytoestrogens that could be beneficial in menopausal and breast feeding women. 3 Aloe Vera Gel Aloe vera The leaf juices of the aloe plant have important medicinal uses. It stimulates collagen synthesis and skin regeneration after a burn; it helps heal acne, improve the appearance of wrinkles and hydrate damaged skin. 4 Apple Malus domestica Other names include apple cider vinegar, wine vinegar. The best of the medicinal compounds are contained in the apple peel. It helps in relieving arthritis, apple contains malic and tartaric acids, salts of potassium, sodium, magnesium and iron. Copyright © 2005-2013 Annie’s Remedy (Anniesremedy.com). Crateva adansonii belonging to the family Capparacea

and phylum Magnoliophyta is small tree of forest and

15savanna woodland, often on river-banks, widely

distributed in Nigeria and across Africa. The leaves are

applied externally to relieve pain in joint, the fresh juice

of leaves is used for the relief of ear-ache, eye infection

and anodyne in toothache. Powder of bark is used in

rheumatism, itch, epilepsy and asthma (Sivarajan &

Balachandran, 1994).

1.2 Crateva adansonii as a plant.

The flowering tree Crateva adansonii is called the

sacred garlic pear and temple plant. The tree is

sometimes called the spider tree because the showy

flowers bear long, spidery stamens. It is native to Japan,

Australia, much of south East Asia and several south

pacific Islands. It grows in forest and savanna woodland,

often on river-banks from Senegal to N Nigeria, and

across Africa to Zaire, Tanganyika and Madagascar. The

plants common name is amakarode in Igbo, In Yoruba,

it is egun-orun and in Hausa, it is ungududu. The tree

16attains approximately 15m height. The trunk is irregular,

seldom straight, but is worthy of cultivation as an

ornamental for its dense masses of white flowers borne

at the ends of all the shoots. In the bush, owing to grass

burning which it survives and repeated stripping of its

leaves, the tree is often stunted. The wood is soft and

yellow and strong-smelling when cut. The leaves are

however eaten in soups or mixed with cereals. They are

boiled and added to mixture called in Hausa “Kwado”

containing a paste of locust beans, Parkia Spp.

(Leguminosae Mimisoideae). The Yoruba consumes the

leaves as a potherb.

In upper volt they are the ingredient of sauces. To

some people the leaves are taken only in time of dearth

and they are sold in northern markets. The bark is

widely used for stomach-troubles in Nigeria. In Jebel

Marra a bark-paste is used as a poultice on swellings.

Crateva adansonii is a decidious plant with three

palmate leaves. The leaves are arranged opposite one

17another. They are elliptic with entire margins. The

flowers are pale green. The plants bloom from March to

May. The flowers are arranged in racemes. The fruits are

berries. It can withstand temperatures above 1oc and

2oc (Burkill, 1985).

Taxonomy

Family: Capparaceae

Genus: Crateva

Species: Crateva adansonii DC

Phylum/Division: Magnoliophyta

Sub division: Magnoliophytina

Classes: Rosopsida

Sub class: Dilleniidae

Super order: Violanae

Order: Capparales

The medicinal effect of Crateva adansonii plant bark

is includes general healing, leprosy, stomach troubles.

For the leaf, it is used in eye treatments, liver, for leaf;

a pain-killer, for roots, dropsy, swellings, edema, gout,

18veneral diseases. Powder of bark is used in rheumatism,

itch, epilepsy and asthma.

1.3 Research Aim and Objectives

Aim:

To study the anti-inflammatory effect of

dichloromethane fraction extracts from Crateva

adansonii on rats.Objectives:

i) To determine the anti-inflammatory effect of

Crateva adansonii extracts in methanol on rats.

ii) To determine the phytochemicals present in

Crateva adansonii extract in methanol on rats.


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